A time-saving and sensitive high resolution method lor the analysis and identification
of platelet glycoproteins using non-radioactive compounds has been developed. Platelet
proteins (50 μg) of normal subjects were separated by isoelectric focusing and SDS-PAGE.
Proteins were either stained with silver or electroblotted onto nitrocellulose. Nitrocellulose
blots were treated with the following biotinylated lectins : Abrus precatorius lectin,
concanavalin A, Lens culinaris lectin, or wheat germ agglutinin. Staining was achieved
by avidin-biotin-coupled peroxidase using 4-chloro-l-naphthol as the substrate. A
rapid overview of platelet glycoproteins may be obtained by applying all the lectins
to a single blot.
Keywords
Platelet glycoproteins - Two-dimensional O’Farrell gel electrophoresis - Western blot
- Avidin-biotin - Lectins